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Graduate Student Seminar - 05/03/2013

"Effects of PCKδ Inhibitiion on Posterior Lateral Line Primordium Migration in Danio reria"

by Deborah Hemingway

Friday, May 3, 2013 -- 12:00 p.m.
Large Conference Room, 1207 Energy Research Facility

Advisor:  Associate Professor Wolfgang Losert

Collective cell migration has diverse occurrences in nature such as wound healing, development, and cancer metastasis. By examining collective cell migration, we can gain insight into the phenomena that affect human health. The fibroblast growth factor (FGF) signaling pathway is part of the method used by collective groups of cells to communicate with each other and is known to affect collective cell migration behaviors.

This in vivo experiment examines the effect of inhibiting PKC*, a protein in the PLCy downstream branch of the FGF signaling pathway. The migration behavior of the posterior lateral line primordium in developing Danio rerio (zebrafish) embryos, a group of collectively moving cells, was analyzed. The inhibition of PKC* was found to significantly alter migration by drastically decreasing the leading edge migration velocity and reducing the collectivity of individual cell migration within the primordium, and that the presence of transverse waves could not be identified on the cellular or primordium levels in either wildtype or transgenic lines.

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